MODULATORS :: APPLICATIONS :: Measurement of Degree of Polarization of Fluorescence/Luminescence

The polarization of fluorescence radiation emitted by a sample is frequently correlated with the (linear) polarization of the exciting radiation.  Usually the emitted light polarization is parallel to the exciting light polarization.  A parameter P (Equation 1) defines the degree of polarization of fluorescent light.

EXPERIMENTAL SETUP



             

Where I_|| = the intensity of fluorescence light parallel to excitation,     I= Intensity of fluorescent light normal to excitation, A₀ = /2 (/4 waves)  and J₁(A₀) = 0.567

ADDITIONAL TOOLS
Fluorescence Complete Solution Packages


ADDITIONAL READING
K.W. Hipps and G.A. Crosby, Applications of the Photoelastic Modulator to Polarization Spectroscopy, J. Phys. Chem 83, 555-562 (1979).